Materials and Methods
Materials and Methods
Specimens of Phyllomys consist of skins, skulls, post-cranial skeletons, and fluid-preserved material housed. Specimens used in the molecular analyses were collected in the field during several expeditions led by different research groups. Specimens were collected using Sherman, Tomahawk, or locally made live traps placed either on the ground or in the tree branches and vines. Some were collected during night censuses, using a .410-gauge shotgun loaded with dust shot (#12), and others were caught by hand from nests in tree hollows during the day. Voucher specimens were preserved as study skins and skulls or in fluid, following standard museum procedures. Liver samples were preserved in ethyl alcohol and liquid nitrogen from DNA analyses. Two-way analyses of variance (ANOVA) were used to examine non-geographic variation (due to sex and age). Principal components analyses (PCA) were used to analyze how individual measurements contribute to the total pool of variation.
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