Jump to ContentJump to Main Navigation
Emerging Avian Disease$
Users without a subscription are not able to see the full content.

Ellen Paul

Print publication date: 2012

Print ISBN-13: 9780520272378

Published to California Scholarship Online: September 2012

DOI: 10.1525/california/9780520272378.001.0001

Show Summary Details
Page of

PRINTED FROM CALIFORNIA SCHOLARSHIP ONLINE (www.california.universitypressscholarship.com). (c) Copyright University of California Press, 2022. All Rights Reserved. An individual user may print out a PDF of a single chapter of a monograph in CALSO for personal use.date: 25 May 2022

Immunophenotyping of Avian Lymphocytes

Immunophenotyping of Avian Lymphocytes

Implications and Future for Understanding Disease in Birds

(p.81) Chapter Seven Immunophenotyping of Avian Lymphocytes
Emerging Avian Disease

Jeanne M. Fair

Kirsten J. Taylor-McCabe

Yulin Shou

Babetta L. Marrone

University of California Press

Chicken (Gallus gallus domesticus)T-cell populations can be delineated into subsets based on their expression of cell-surface proteins such as cluster of differentiation (CD) cell surface markers. However, immunophenotyping using flow cytometry in birds has focused on cell characterization in the thymus and spleen during development in chickens. West Nile virus (WNV) causes differential infections in birds, ranging the entire spectrum of pathogenesis. In order to accurately assess immunocompetence to diseases such as WNV in birds, more efficient methodology to access natural variability in avian immune function must be devised and understood. Previously, lymphocyte subpopulations CD4+ and CD8+ have been found to be critical for clearing infection of WNV in mammals. Focusing on chickens, a species that is susceptible but not infective for WNV, our objectives were to: (1) further develop flow cytometry for estimating subpopulations of lymphocytes in peripheral blood from poultry, (2) estimate the best antibody and cell marker combination for estimating lymphocyte subpopulations, and (3) estimate repeatability and application to other avian species susceptible to WNV. Immunophenotyping of CD3+, CD4+, CD8+, and CD45+ was successfully completed for chicken peripheral blood but not for the Common Raven (Corvus corax) or Black-billed Magpie (Pica hudsonia). Future studies include immunophenotyping during infection studies of WNV in chickens and further development of flow cytometry for other bird species.

Keywords:   chicken, flow cytometry, Gallus gallus domesticus, host range, immunophenotyping, lymphocytes, West Nile virus

California Scholarship Online requires a subscription or purchase to access the full text of books within the service. Public users can however freely search the site and view the abstracts and keywords for each book and chapter.

Please, subscribe or login to access full text content.

If you think you should have access to this title, please contact your librarian.

To troubleshoot, please check our FAQs, and if you can't find the answer there, please contact us.